While the initial development phase of Doppler Global Velocimetry (DGV) has been successfully completed, there remains a critical next phase to be conducted, namely the determination of an error budget to provide quantitative bounds for measurements obtained by this technology. This paper describes a laboratory investigation that consisted of a detailed interrogation of potential error sources to determine their contribution to the overall DGV error budget. A few sources of error were obvious; e.g., iodine vapor adsorption lines, optical systems, and camera characteristics. However, additional non-obvious sources were also discovered; e.g., laser frequency and single-frequency stability, media scattering characteristics, and interference fringes. This paper describes each identified error source, its effect on the overall error budget, and where possible, corrective procedures to reduce or eliminate its effect.